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EU Marie Curie Chair
Birgit Arnholdt-Schmitt
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Competence Focus: "Functional Cell Reprogramming and Organism Plasticity (FunCrop)" created in May 2016 as a SMART extension of the EU Marie Curie Chair  

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Selection of Reference Genes for Transcription Studies on Adventitious Root Induction in Olive (Olea Europaea L.) Microshoots Considering Co-expression and Average Transcriptional Stability
Publication Type
Article (Pre-print)
Authors

Carlos Noceda, Augusto Peixe, Birgit Arnholdt-Schmitt

Year
2020
Document
application/pdf
[aec50abc-721a-4fe8-97ab-9656e5792472.pdf]
(1.55 MB)
Abstract

Selection of reference genes (RGs) for normalization of PCR-gene expression data includes two crucial steps: determination of the among-sample transcriptionally more stable genes and subsequent choosing of the most suitable genes as internal controls. Both steps can be carried-out through generally accepted strategies each having different strengths and weaknesses. The present study proposes to reinforce normalization of gene expression data by integrating and adding analytical revision at critical steps of those accepted procedures. Especially crucial is to counterbalance a higher representative number of RGs with a correspondent increase in their average transcriptional instability or a generalised co-expression trend among the samples. This methodological study used in vitro olive adventitious rooting as an experimental system, since the underlying morphogenetic process -wich is common to diverse species- is still not completely understood.

Palavras Chave
RT-qPCR, reference genes, expression stability, co-regulation, olive, adventitious rooting, alternative oxidase
Editora
Research Square
Link
https://doi.org/10.21203/rs.3.rs-60001/v1

Citation

Carlos Noceda, Augusto Peixe, Birgit Arnholdt-Schmitt. Selection of Reference Genes for Transcription Studies on Adventitious Root Induction in Olive (Olea Europaea L.) Microshoots Considering Co-expression and Average Transcriptional Stability, 19 August 2020, PREPRINT (Version 1) available at Research Square [+https://doi.org/10.21203/rs.3.rs-60001/v1+]

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